Chopping up Plasmids

Learning Outcomes

After completing this module you should be able to:

  • Describe the use of restriction enzymes to cut plasmid DNA in smaller pieces (fragments)
  • Explain how separation of DNA fragments in gel electrophoresis is based on their size
  • Analyse and interpret experimental data obtained from gel electrophoresis of plasmids
1: Three thieves and a beaded necklace
2: And the moral is?
3: A real plasmid
4: Practice with restriction sites
5: What's wrong with these gels?
6: Playing with plasmids
7: Other weird things
8: Summary