It is exactly the same in the lab–try it yourself
John and Suzy want to measure the concentrations of a protein (Bovine Serum Albumin, BSA) in solution. After finding the proper wavelength (280nm), the ODs of 1 mg/mL, 2 mg/mL, 4 mg/mL, and 8 mg/mL BSA solutions were obtained. They then measured the ODs of two unknown BSA solutions. What were the concentrations of the protein solutions?
BSA concentration (mg/mL) |
OD |
---|---|
1 |
0.3 |
2 |
0.6 |
4 |
1.2 |
8 |
2.4 |
Unknown 1 |
0.7 |
Unknown 2 |
1.5 |
First, what is e?
So far, we know that e = 0.3 OD/mg/mL. Fitting that into our equation, we get
OD = 0.3 c
Now that you have e, what are the concentrations of the two unknown protein samples? (Their ODs were 0.7 and 1.5).